All information in this guide concerning the orbitrap elite mass. The thermo ltq orbitrap velos mass spectrometer is a hybrid mass spectrometer incorporating the ltq velos dual cell linear trap and the orbitrap analyzer. Tuning adjusts the voltages on the source and ion optics to maximize the instruments sensitivity. Color index disperse yellow 42 dy42, a highvolume disperse dye for polyester, was used to compare the capabilities of the ltq orbitrap xl and the ltq fticr with respect to mass measurement accuracy mma, spectral accuracy, and sulfur counting. Untargeted metabolomics workflow using uhplcquadrupole. Editors, technical documentation thermo electron bremen gmbh advanced mass spectrometry hannakunathstr. Xray photoelectron spectroscopy kratos axis supra xps. It is the ultimate protein identification and biomarker discovery platform featuring unsurpassed sensitivity in ms and msn, rapid scan rate, high mass accuracy and up to 100k resolution power. User manuals user manuals are part of the delivery scope. Users will gain handson experience using both esi and apci ionization techniques and learn the benefits of. The ltq orbitrap elite with waters nanoacquity is broadly used for.
Thermo scientific ltq orbitrap velos hy b rid ft mass spectrometer performance speci fications performance characteristics. Mass spectrometer thermo scientific ltq xl linear ion. Thermo scientific ltq orbitrap velos hy b rid ft mass. Ltq orbitrap xl etd hardware manual thermo fisher scientific. The ltq xl has msn capabilities, which is a powerful analytical tool for use in structural analysis. The thermo scientific exactive plus emr is a orbitrap based electrostatic ion trap mass. Has anyone replaced the turbopump in an ltq xl mass spectrometer. Thermo scientific ltq xl getting started pdf download. Ltq orbitrap xl hardware manual or ltq orbitrap xl etd. Thermo finnigan ltq msms ion max esi source gentech. Ltq orbitrap series preinstallation requirements planet orbitrap. Wheels at the bottom side of the instrument facilitate positioning the ltq orbitrap xl at the intended place in the laboratory. The thermo scientific ltq xl linear ion trap mass spectrometer provides accurate mass measurements mz 504000 with resolution of 60,000 and high sensitivity low fetomole to.
Refurbished thermo orbitrap systems gentechscientific. Ltq orbitrap velos getting started concordia university. The invention of the orbitrap analyzer and its proofofprinciple by makarov at the end of the 1990s started a sequence of technology improvements which resulted in the commercial introduction of this analyzer by thermo fisher scientific as a part of the hybrid ltq orbitrap instrument in 2005. Thermo scientific ltq xl manuals manuals and user guides for thermo scientific ltq xl. The thermo scientific ltq xl is a linear ion trap lit mass spectrometer with electrospray ionization esi and atmospheric pressure chemical ionization apci capabilities. Changes to the manual ii ltq orbitrap preinstallation requirements guide thermo electron corporation changes to the manual to suggest changes to this manual, please send your comments to. Find new and used lab equipment and shop a wide selection of products and vendors. Troubleshooting ltq and ltqorbitrap lcmsms systems with massqc.
The ltq xl ion trap mass spectrometer with agilent 1100 hplc system is used for a variety of studies including metabolite identification, stability testing, degradation products, and highabundance quantitation. In msms mode the linear ion trap only retains a chosen mass window. Product specifications new api source with new slens ion optics technology. Details about thermo scientific finnigan mass spectrometer ltq orbitrap xl. All information in this guide concerning the orbitrap. Schematic of the ltq orbitrap xl mass spectrometer with an hcd collision cell. Ltq orbitrap xl hybrid ion trap orbitrap mass spectrometer support troubleshooting find valuable information. Maldi source hardware manual with the maldi ltq xl system.
The thermo finnigan ltq is a stateoftheart, segmented linear ion trap mass spectrometer. Ltq series hardware manual thermo fisher scientific. This ltq xl getting started manual provides information on how to set up, calibrate, and. Ltq orbitrap operations 4 days this course is intended for new users to the thermo scientific orbitrap system.
Mass spectrometrybased proteomics using q exactive, a. The ltq orbitrap xl features an hcd collision cell to provide additional. We have 2 thermo scientific ltq xl manuals available for free pdf download. This manual should be kept near the instrument to be available for quick reference. The new maldi source, available on the thermo scientific ltq orbitrap xl and ltq orbitrap discovery, is ideally suited for proteomic and metabolism applications. The ltq orbitrap xl is a member of the family of ltq mass spectrometer ms hybrid instruments. Thermo scientific ltq orbitrap xl the most confident protein identification. Service your orbitrap from circuit boards to complete bench repair we can return your systems to optimal performance. Calibration sets the correct values on the mass spectrometer components to produce a proper qualitative spectrum. Pulsed q dissociation pqd for generating more fragments and extending the low mass range. However, the ltq orbitrap velos is equipped with electron multipliers, which allows scaling of the number of ions in a direct manner. About this guide this ltq orbitrap xl hardware manual contains a description of the modes of operation and principle hardware components of your ltq orbitrap xl instrument.
Superior mass accuracy, mass resolution, dynamic range, detection power, high productivity, and low maintenance. In addition, this manual provides stepbystep instructions for. For calibration, the user can change this value to 60% to make more 8 which may make calibration easier to pass. Ltq orbitrap xl hybrid ion traporbitrap mass spectrometer. Mass spectrometers department of chemistry mellon college. A triple quadrupole adds selected reaction monitoring. Thermo fisher ltq orbitrap xl ion trap orbitrap mass spectrometer. The new ltq orbitrap xl hybrid ft mass spectrometer combines a linear ion trap ms and the orbitrap mass analyzer. Mass spectrometer thermo scientific ltq orbitrap velos. With its revolutionarily new, miniature design, orbitrap combines high speed with excellent quantification properties, ranking favorably in many analytical applications. The thermo scientific ltq orbitrap xl hybrid ftms fourier transform mass spectrometer supports a wide range of applications from routine compound identification to the most challenging analysis of low level components in complex mixtures. Untargeted metabolomics workflow using uhplcquadrupole orbitrap mass spectrometer and sieve 2.
Rent orbitraps with competitive rates and flexible payment plans quickly add highresolution accuratemass analysis to your lab. Superior resolution of agilent 6540 uhd qtof over thermo ltq orbitrap xl for fast uhplc applications abstract mass spectral resolution is undeniably valuable and is an important decision criterion in the choice of an lcms system for accuratemass analyses. Scope of this guide ltq orbitrap xl getting started includes the following chapters. Ltq orbitrap velos is a member of the family of ltq mass spectrometer ms detectors. The new hcd cell in the ltq orbitrap velos is more efficient, resulting in improved quantitation of isobarically labeled peptides, such as applications requiring tandem mass tags tmt. Demonstrate a generic, integrated workflow for untargeted metabolomics study using a uhplcbenchtop thermo scientific orbitrap mass spectrometer and informatics software.
Orbitrap discovery and xl or an additional linear octopole located at the rear end of the c. Ltq orbitrap xl, discovery, classic calibration solutions. Scope of this guide this ltq orbitrap hardware manual contains a description of the modes of. Ltq orbitrap xl hardware manual thermo fisher scientific. Instrument configuration gives instructions about configuring your. We know you, or someone you know, worked very hard to get your orbitrap based mass spectrometer in your lab and even harder preparing your precious samples. The data was generated by hplc apparatus agilent 1200 series, ltq orbitrap xl thermofisher scientific. Principle of operation the hybrid ft mass spectrometer combines a linear ion trap ms and the orbitrap mass analyzer. Ltq orbitrap hardware manual thermo fisher scientific. An orbitrap analyzer for fourier transformation b ased analysis. Orbitrap xl, which provides additional ms n versatility. Superior resolution of agilent 6540 uhd qtof over thermo ltq. Testing the operation of the ms detector in the esims mode 52. Jun 03, 2011 target values on q exactive were similar to those typically used on an ltq orbitrap velos.
Orbitrap fusion series mass spectrometer we know you, or someone you know, worked very hard to get your orbitrap based mass spectrometer in your lab and even harder preparing your precious samples. The symbol q represents a quadrupole mass analyzer, q is a radio frequency collision quadrupole, tof is a timeofflight mass spectrometer, b is a magnetic sector and e is an electric sector. Resources for optimal performance of your orbitrap fusion series mass spectrometer. We are looking for more detailed instructions to do this than what is included in the ltq xl hardware manual and would appreciate. The high mass accuracy of the ltq orbitrap velos increases the speed and con.
Automatic gain control, foundation, ion maxs, ltq xl, orbitrap elite. Offlease 2008 thermo ltq orbitrap discovery with ionmax, esi, apci, chiller, xcalibur data system, powervar ups if you would like a shipping quote, please give us your location. This course covers the fundamentals of mass spectrometry, with emphasis on small molecule accurate mass applications. A single quadrupole offers selected ion monitoring. The ltq orbitrap instruments represent a multistage trap combination fig. Equipped with an electrospray ionization esi source, the ltq boasts improved capacity and trapping efficiency, higher scan speed and increased sensitivity. Mass resolution allows the separate identification of nearisobaric components that. The sample is evacuated to 109 torr or better, for quality measurements. Determination of the target value is based on predictive automatic gain control pagc in both instruments. A linear ion trap thermo scientific ltq xl for sample. The different mz separation elements of a hybrid mass spectrometer can be represented by a shorthand notation. We are working just as hard to make sure that you have the latest information and all the additional resources you need to produce the highest. A maldi ltq orbitrap xl mass spectrometer for bioimaging.
It is the ultimate protein identification and biomarker discovery platform featuring unsurpassed sensitivity in ms and msn, rapid scan rate, high mass accuracy and. As demonstrated in many recent publications, the orbitrap mass analyzer supports a wide range of applications. Ltq xl is the only mass spectrometer that offers multiple dissociation techniques, pqd, etd and cid. This ltq orbitrap xl etd hardware manual is intended for all. Page 42 45 degrees counterclockwise to free the probe from the alignment notch. Ltq, lxq, orbitrap, thermo scientific, and xcalibur are registered trademarks of thermo fisher. Warning symbols alert the user to check for hazardous conditions. The kratos axis supra xps xray photoelectron spectroscopy system offers surface analysis, surface chemical mapping, surface imaging, and depth profiling of metallic, semimetallic and nonmetallic samples as deep as 1 nm. Hardware manual revision a orbitrap tribrid series preinstallation. Welcome to the thermo scientific, ltq orbitrap xl system. Thermo scientific ltq xl getting started 176 pages brand.
This talk will cover the calibration, tuning, and operation of the ltqorbitrap series of instruments. Has anyone replaced the turbopump in an ltq xl mass. Spectral accuracy and sulfur counting capabilities of the. View and download thermo scientific etd getting started manual online. Shop a wide selection of thermo scientific ltq xl linear ion trap mass spectrometers on labx. Ltq orbitrap classic, was introduced by thermo electron currently thermo fisher scienti. With this proposal, we are requesting funds to acquire a maldi ltq orbitrap xl mass spectrometer from thermo scientific for bioimaging at the public health research institute phri, which will be used under biosafety level 3 bsl3 biocontainment within the rutgers regional biocontainment laboratory rbl. Thermo ltq orbitrap xl unsw mark wainwright analytical. Resources for optimal performance of your orbitrap elite mass spectrometer. If you need additional guidance, refer to ltq xl online help, ltq xl getting connected, ion max api source hardware manual, or the ltq xl hardware manual. A collision cell for performing higher energy cid experiments.
Thermo scientific finnigan mass spectrometer ltq orbitrap xl. Ltq orbitrap xl hybrid ion traporbitrap mass spectrometer support orbitrap fusion. Thermo scientific ltq xl high performance linear ion trap delivers more structural information than any other ion trap mass spectrometer. Unparalleled sensitivity and unmatched msnspectral quality. This ltq orbitrap velos getting started manual is intended for all personnel that. Thermo scientific ltq orbitrap xl from thermo fisher. Thermo scientific finnigan mass spectrometer ltq orbitrap. Please be aware that manuals are shipped online on the instrument computer. Dartthermo ltq orbitrap interface manual for thermo ltq orbitrap velos, ltq orbitrap xl, ltq ion trap, exactive high performance, and all other instruments that utilize the ion max source version 20100814 ionsense inc. Ltq xl linear ion trap mass spectrometer thermo scientific. This is being shipped through a freight company because of the size and fragility of the instrument. Instrument configuration gives instructions about configuring the. The new maldi source, available on the thermo scientific ltq orbitrap xl and ltq orbitrap discovery, is. After processing peaks with xcms online tool i did annotations with different databases.
This ltq orbitrap velos hardware manual contains a description of the modes of operation. Thermo scientific ltq xl linear ion trap mass spectrometer. Note the following procedures assume that you are familiar with your instrument and software. Thermo scientific ltq orbitrap xl linear ion trap mass. Should you need a replacement for current or older leybold products, here are the most common manuals. About this guide this ltq orbitrap velos getting started manual provides information on how to set up, calibrate, and tune the ltq orbitrap velos. Troubleshooting liquid chromatography tandem mass spectrometry lc msms systems is a top priority in academic and commercial proteomics laboratories. Easynlc 1200 user s manual please read prior to nlcms inquiries. For the ltq xl and ltq velos ms detectors compatible with ltq orbitrap xl and ltq orbitrap velos.
The instrument is designed to be placed with it s rear panel against a wall. Obtain high sensitivity full scan ms along with indepth msn crm capabilities with the thermo scientific ltq xl linear ion trap mass spectrometer. Use of this web site constitutes acceptance of the labx user. Thermo scientific maldi ltq orbitrap series simplifies the analysis of whole tissue, biological and polymer samples without extensive sample preparation. Thermo fisher scientific orbitrap elite hardware manual pn 1288170, revision a i read this first welcome to the thermo scientific or bitrap elite system. Ltq orbitrap xl hardware manual, ltq orbitrap xl etd. Subzero max pro medical freeze spray can 10oz us made.
Do not change the external or internal grounding connections. Send us an email if you are looking for a specific resource or advice on how you can get the most out of your instrument. Tampering with or disconnecting these connections could endanger you. Thermo ltq orbitrap xl the orbitrap velos xl is based at lower campus and is used for nonbiological samples. Procedures in chapters 14 can be pe rformed from the xcalibur tune plus window. The orbitrap xl supports a wide range of applications from routine compound identification to analysis of lowlevel components in complex mixtures. Spectral accuracy and sulfur counting capabilities of the ltqft. Extended warranty extend your peace of mind with our extended warranty. Ltq series getting started guide thermo fisher scientific. Who uses this guide this ltq orbitrap xl hardware manual is intended for all personnel that need a thorough understanding of the instrument to perform maintenance or troubleshooting. Ions generated by api are collected in the ltq xl followed by axial ejection to the cshaped storage trap which is used to store and collisionally. Etd module hardware manual for the ltq xl and ltq velos ms detectors compatible with ltq orbitrap xl and ltq orbitrap velos. Thermo scientific ltq orbitrap xl gentech scientific.
Also, this symbol appears on the instrument to refer the user to instructions in this manual. The ltq xl extends the legendary msnperformance of the ltq ion trap, incorporating more techniques to generate structural information through innovations in thermo scientific ion trap technology. Ions can be selected in the linear ion trap and fragmented either in the ion trap cid or in the hcd collision cell. Sep 21, 2011 color index disperse yellow 42 dy42, a highvolume disperse dye for polyester, was used to compare the capabilities of the ltq orbitrap xl and the ltq fticr with respect to mass measurement accuracy mma, spectral accuracy, and sulfur counting. In ms mode the linear trap performs the function of collecting the ion population, passing them on to an intermediate ctrap for injection and analysis in the orbitrap analyzer at high resolution. For all related user manuals and quick references, click operator manuals. Ltq orbitrap velos hardware manual brown university. Orbitrap fusion tribrid mass spectrometer support thermo.
Thank you for watching gentech scientific visit our site. The orbitrap elite is a member of the family of ltq mass spectrometer ms hybrid instruments. Ltq orbitrap xl offering outstanding mass accuracy, resolving power, dynamic range, and high sensitivity the hybrid ft mass spectrometer combines a linear ion trap ms and the orbitrap mass analyzer. Metabolomic analysis of green and black tea extracts using an ltq orbitrap xl hybrid linear ion trap mass spectrometer donna l. Spectral accuracy and sulfur counting capabilities of the ltq. The results of this research will be used in the construction of a dye database for forensic purposes. Mass spectrometrybased proteomics using q exactive, a high. Ltq xl linear iontrap ms user s manual please read for training. Orbitrap is the newest addition to the family of highresolution mass spectrometry analyzers. Orbitrap mass analysers are recognised for their ability to produce ultra high mass accuracy and resolution measurements. The ltq orbitrap xl is the comprehensive proteomics solution for protein id, characterization and quantitation.
This ltq orbitrap xl getting started manual is intended for all personnel that need to operate the ltq orbitrap xl, especially the key operator. We present a new software tool, massqc, that builds on work from the national institute of standards and technology nist to provide an easytouse and powerful method to evaluate and track lc. Initial ltqorbitrap xl data showed mmas greater than 3 ppm and. In addition, precursor ions can be fragmented in the c.
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